CHLC Report

Cooperative Human Linkage Center
Volume 3, Number 1: October, 1995

UPDATE

In September, 1994 the CHLC, in collaboration with Genethon, the University of Utah, and Yale University published a new level of human linkage map unprecedented both in the number of markers it contains and the extent of collaborative interactions that made it possible. This map included over 5,800 DNA polymorphisms typed on the CEPH reference family and placed into genetic map locations. Almost 1,000 of these markers were placed into a high odds map, and elements of this map then used as a framework into which an additional, almost 5,000 markers were placed into bin or best location positions. The markers included over 3,600 sequence-based (STS class) polymorphisms as well as over 400 genes. These efforts were made possible through the international CEPH collaboration established over ten years ago by Professor Dausset and which has provided the benchmark resource for human genetic linkage mapping since. The efforts of Drs. Dausset, Cohen, Cann and the CEPH staff over this decade have made it possible for the 110 CEPH collaborators around the world to generate genotypic information which provided the basic substrate for this map construction.

Since that time, we have placed into the genetic maps an additional nine hundred high-heterozygosity, CHLC-class markers and mapped by somatic cell hybrids a total of 2,400 tri- and tetranucleotide markers. This work was published in two articles appearing in Human Molecular Genetics this month (Gastier et al., 4(10):1829; Sheffield et al., 4(10):1837). Attached to this report is the overview of these map locations, as updated from the original publication one year ago.

More importantly, the specifics of these markers and the placement of each marker into either well-defined order or into a genetic bin is available via our various e-mail, ftp, and World Wide Web sites. These are listed in the table below, along with other relevant gene mapping, electronic information addresses.

These markers continue to prove their use for both genetic and physical mapping projects and now provide a degree of available genetic markers of unprecedented quality and density. We continue to explore the behavior of these markers with respect to recombination and physical location and continue to develop such markers to generate even more powerful maps to be used in the localization of genetic disease. Additional information about maps and markers can be obtained at the e-mail addresses below.

The maps shown here have strong support for the orders as outlined with bins shown in cM. The numbers in the histogram boxes to the left are numbers of additional CHLC tri/tetra markers in that bin. Other markers in those bins can be seen on maps on our WWW sites. As noted above, we also have over 2,000 additional CHLC markers mapped by chromosome assignment.

Jeffrey C. Murray, M.D.
Principal Investigator, CHLC
The University of Iowa
Iowa City, IA 52242
TEL: (319) 335-6897
FAX: (319) 335-6970
jeff-murray@uiowa.edu 

            CHLC     E-mail: info-server@chlc.org
                     FTP: ftp.chlc.org
                     WWW: http://www.chlc.org 

         Genethon    FTP: ftp.genethon.fr
                     WWW: http://www.genethon.fr

            GDB      E-mail: help@gdb.org
                     FTP: ftp.gdb.org
                     WWW: http://gdbwww.gdb.org

        Jackson Lab  E-mail: mgi-help@informatics.jax.org
                     FTP:  ftp.informatics.jax.org
                     WWW: http://www.jax.org

            CEPH     E-mail: ceph-genethon-map@cephb.fr
                     FTP: ceph-genethon-map.genethon.fr
                     WWW: http://www.cephb.fr/bio/ceph-genethon-map.html

         Whitehead   E-mail: genome_database@genome.wi.mit.edu
                     FTP: genome.wi.mit.edu
                     WWW: http://www-genome.wi.mit.edu/

Last modified: Fri Nov 3 09:30:25 EST 1995

The CHLC Informatics Group (help@chlc.org)