AAC        AAG        AAT        ACC        ACT        AGC       AGG        ATC
                                                       
Total Clones                50         70         50         51         189        50        60         61
                                                               
Primers designed         13 (26%)   12 (17%)   21 (42%)   10 (20%)   22 (12%)    9 (18%)   9 (15%)   24 (39%)
No primers-ALU/LINE ^    20 (40%)   12 (17%)   16 (32%)    1 (2%)     5 (3%)     2 (4%)    1 (2%)     1 (2%)
No suitable primers       0 (0%)     1 (1%)     1 (2%)     1 (2%)     6 (3%)     2 (4%)    0 (0%)     0 (0%)
Misplaced repeat *       13 (26%)    8 (11%)    3 (6%)     6 (12%    22 (12%)   12 (24%)  13 (22%)   10 (16%)
Repeat length <7          1 (2%)    13 (19%)    3 (6%)    12 (24%)   45 (24%)   16 (32%)  31 (52%)   16 (26%)
Poor sequence             1 (2%)     4 (6%)     4 (8%)     9 (18%)    7 (4%)     2 (4%)    4 (7%)     5 (8%)
Duplicates                2 (4%)    20 (29%)    2 (4%)    12 (24%)   82 (43%)    7 (14%)   2 (3%)     5 (8%)

^ Repetitive element prevented the design of unique primers flanking the 
  observed trinucleotide repeat

* Repeats which were too close to the vector to have primers designed or 
  too distant from the vector to be accessible by sequencing